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KMID : 1377020190160020191
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Tissue Engineering and Regenerative Medicine
2019 Volume.16 No. 2 p.191 ~ p.200
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Treatment of Periodontal Bone Defects with Stem Cells from Inflammatory Dental Pulp Tissues in Miniature Swine
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Li Ye
Nan Xi
Zhong Tian-Yu
Li Tong
Li Ang
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Abstract
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Background: Containing a certain proportion of mesenchymal stem cells, inflammatory dental tissue showed great tissue regeneration potential in recent years. However, whether it is applicable to promote tissue regeneration in vivo remains to be elucidated. Therefore, we evaluated the feasibility of stem cells from inflammatory dental pulp tissues (DPSCs-IPs) to reconstruct periodontal defects in miniature pigs.
Methods: The autologous pig DPSCs-IPs were first cultured, appraised and loaded onto β-tricalcium phosphate (β-TCP). The compounds were then engrafted into an artificially-created periodontal defect. Three months later, the extent of periodontal regeneration was evaluated. Clinical examination, radiological examination and immunohistochemical staining were used to assess periodontal regeneration.
Results: The data collectively showed that DPSCs-IPs from miniature pigs expressed moderate to high levels of STRO-1 and CD146 as well as low levels of CD34 and CD45. DPSCs-IPs have osteogentic, adipogenic and chondrogenic differentiation abilities. DPSCs-IPs were engrafted onto β-TCP and regenerated bone to repair periodontal defects by 3 months’ post-surgical reconstruction.
Conclusion: Autologous DPSCs-IPs may be a feasible means of periodontal regeneration in miniature pigs.
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KEYWORD
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Dental pulp tissue, Bone defeats, Stem cells, Periodontitis
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